In accordance with the present invention there is provided a method for the separation
of a triglycoalkaloid in which the triglycoside portion comprises -L-rhamnopyranosyl-(12gal)-O--D-glucopyranosyl-(13gal)--D-galactopyranose
(or 6-deoxy--L-mannopyranosyl-(12)-O-[-D-glucopyranosyl-(13)]--D-galactopyranoside)
('an rhamnose-glucose-galactose triglycoalkaloid') from a triglycoalkaloid in which
the triglycoside portion is -L-rhamnopyranosyl-(12glu)-O--L-rhamnopyranosyl-(14glu)--D-glucopyranose
('a rhamnose-rhamnose-glucose triglycoalkaloid') in a mixture containing both,
the method comprising the steps of:
Combining the mixture with a portion of alcoholic solvent;
Causing or allowing a substantial portion of the rhamnose-rhamnose-glucose
triglycoalkaloid to dissolve, thereby generating an alcoholic solution substantially
of the rhamnose-rhamnose-glucose triglycoalkaloid, and a solid substantially comprising
the rhamnose-glucose-galactose triglycoalkaloid; and
Separating the solid substantially comprising the rhamnose-glucose-galactose
triglycoalkaloid from the solution substantially of the rhamnose-rhamnose-glucose triglycoalkaloid.