A device for conducting an assay to determine the presence or amount of an
analyte in a fluid sample. The device includes a flow path that
facilitates fluidic flow by capillary action. The flow path has a first
fluid permeable region, a second fluid permeable region, a barrier that
inhibits fluidic flow between the first and second regions, and a fluidic
bridge. The bridge includes a fluid permeable material overlapping at
least a portion of each of the first region, the barrier and the second
region.