An article, process, and method for surface plasmon resonance plates are
described. A substrate is covered with a thin metal film onto which a
second thin metal film is deposited. The surface of the second thin metal
film is converted to the metal oxide which is used to covalently bond
organosilanes to the surface. Reactive organosilanes containing terminal
bonding groups are arranged in a plurality of spots that are surrounded
by inert organosilanes. Biomolecule attachment to the binding group is
detected or measured from surface plasmon signals from the first thin
metal film.