A process of parthenogenic activation of mammalian oocytes which includes
increasing intracellular levels of divalent cations in the oocyte; and
reducing phosphorylation of cellular proteins in the oocyte. One method of
accomplishing this is by introducing Ca.sup.2+ free cation, such as
ionomycin, to the oocyte and then preventing phosphorylation of the
cellular proteins within the oocyte by adding a serine-threonine kinase
inhibitor, such as 6-dimethylaminopurine (DMAP).
Um processo da ativação parthenogenic de oocytes mammalian que inclui níveis intracellular crescentes de cations divalent no oocyte; e reduzindo o phosphorylation de proteínas celulares no oocyte. Um método de realizar isto é introduzindo o cation de Ca.sup.2+ livre, tal como o ionomycin, ao oocyte e então impedindo o phosphorylation das proteínas celulares dentro do oocyte adicionando um inibidor do kinase do serine-serine-threonine, tal como 6-dimethylaminopurine (DMAP).