Mass spectrometric, absorbance spectroscopic and fluorescence spectroscopic processes are disclosed to detect the depolymerization of a nucleic acid hybrid in order to qualitatively and quantitatively assay for the presence of a predetermined nucleic acid target. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like, including multiplexed assays.

Spectrometric maciços, o absorbance spectroscopic e os processos spectroscopic do fluorescence são divulgados para detectar qualitatively e quantitatively o depolymerization de um híbrido do ácido nucleic assay para a presença de um alvo predeterminado do ácido nucleic. As aplicações daqueles processos incluem a deteção de únicos polymorphisms do nucleotide, identificação das únicas mudanças da base, speciation, determinação da carga viral, genotyping, diagnóstico médico do marcador, e o gosto, including assays multiplexed.