A method of transforming rice is disclosed. The method begins with the
preparation of copies of a nucleic acid construct that are coated onto
biologically inert carrier particles. In one embodiment, the nucleic
acid-coated carrier particles are physically accelerated toward immature
rice embryos. In another embodiment, the nucleic acid-coated carrier
particles are accelerated toward discs excised from the meristem region of
a rice seedling. Both the bombarded embryos and discs are cultivated to
produce shoots. These shoots are cultivated into whole sexually mature
plants, some of which are transformed. The presence of the nucleic acid
construct is verified in either the shoots or the sexually mature plants.
A particularly advantageous embodiment of the invention is a transformed
Indica rice plant.