An optical fiber holder 1B is an optical fiber holder for holding a plurality of optical fibers in line, and includes a base member 20 with a plurality of parallel positioning grooves 90 for positioning and aligning the plurality of optical fibers, and a lid member 32 adapted to cover the plurality of positioning grooves 90 and to fix the plurality of optical fibers, arranged in the positioning grooves 90, by pressing against the bottom portions of the positioning grooves 90. The lid member 32 has small projections 102 on its portion which comes into contact with the optical fibers.