The present disclosure teaches that the proteins encoded by the BRCA1 and
BRCA2 genes are found in the milk fat globule membranes from humans and
cows. Therefore, BRCA1 and BRCA2 proteins can be isolated from milk
produced by lactating animals. The level of expression of BRCA1 or BRCA2
can be determined by sampling the levels of BRCA1 or BRCA2 proteins found
in the MFGM of lactating animals. The present invention also includes a
method of determining the likelihood that a woman will develop breast
cancer by measuring the amount of the BRCA1 or BRCA2 expression during
lactation. The detection of expression of BRCA1 or BRCA2 can be
accomplished with an antibody raised specifically against BRCA1 or BRCA2
proteins, by isolating BRCA1 or BRCA2 from the milk fat globule membranes
or by detecting activity of BRCA1 or BRCA2. The level of BRCA1 or BRCA2 is
compared to a reference scale of propensity for breast cancer development
correlated to normally active BRCA1 or BRCA2 levels. BRCA1, BRCA2, fatty
acid binding proteins and phosphorylated proteins are found in the MFGM
and the MFGM can be isolated from milk and provided in a form suitable for
oral consumption, i.e. a tablet or capsule of separated milk fat globule
membranes, a food additive, or a fortified dairy commodity.