The present invention describes a method for identifying one or more of a
plurality of sequences differing by one or more single base changes,
insertions, deletions, or translocations in a plurality of target
nucleotide sequences. The ligation phase utilizes a ligation detection
reaction between one oligonucleotide probe, which has a target
sequence-specific portion and an addressable array-specific portion, and a
second oligonucleotide probe, having a target sequence-specific portion
and a detectable label. After the ligation phase, the capture phase is
carried out by hybridizing the ligated oligonucleotide probes to a solid
support with an array of immobilized capture oligonucleotides at least
some of which are complementary to the addressable array-specific portion.
Following completion of the capture phase, a detection phase is carried
out to detect the labels of ligated oligonucleotide probes hybridized to
the solid support. The ligation phase can be preceded by an amplification
process. The present invention also relates to a kit for practicing this
method, a method of forming arrays on solid supports, and the supports
themselves.