The present invention relates to methods for determining nucleic acid sequences that encode components of signal transduction pathways in higher plants. The method comprises combining a portion of an AOX promoter linked in operable fashion to a reporter gene to detect nucleic acid sequences of components of the signal transduction pathways between mitochondria function and metabolic status and nuclear gene expression and the signal transduction pathways between branched chain amino acid biosynthetic pathways and nuclear gene expression. A polynucleotide that encodes a portion of an AOX promoter, AOX1a, operably linked to a luciferase reporter gene is provided. A recombinant vector, transformed cells, and transformed organisms containing this polynucleotide are disclosed.

A invenção atual relaciona-se aos métodos para determinar as seqüências do ácido nucleic que codificam componentes de pathways do transduction do sinal em umas plantas mais elevadas. O método compreende combinar uma parcela de um promoter de AOX ligado na forma operável a um gene do repórter para detectar seqüências do ácido nucleic dos componentes dos pathways do transduction do sinal entre os mitochondria funciona e status metabolic e expressão nuclear do gene e os pathways do transduction do sinal entre pathways biosynthetic ramificados do amino-ácido chain e a expressão nuclear do gene. Um polynucleotide que codifique uma parcela de um promoter de AOX, AOX1a, ligado operably a um gene do repórter do luciferase é fornecido. Um vetor recombinant, umas pilhas transformadas, e uns organismos transformados que contêm este polynucleotide são divulgados.

 
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