Aconitic acid, an unsaturated tricarboxylic acid, is obtained by
fermentation of an aconitic acid-accumulating strain of Aspergillus
terreus or Aspergillus itaconicus, under aerobic conditions, in a
carbohydrate-containing medium, followed by isolation of the aconitic acid
or a salt thereof. A preferred aconitic acid-accumulating microorganism is
the strain of Aspergillus terreus which is on deposit in the Culture
Collection of the Commonewealth Mycological Institute at Kew, Surrey,
United Kingdom, under Accession No. CMI CC 281924.