The invention provides a replacement heart valve which contains an acellular matrix as a structural scaffold. The scaffold is seeded with isolated myofibroblasts and/or endothelial cells prior to implantation into a recipient mammal.

 
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< Mice comprising engrafted functional human hepatocytes

< Chemical cleaning of biological material

> Scaffold formed of tissue treated to eliminate cellular and cytosolic elements

> Clonal cells and cell lines derived from C3A cells and methods of making and using them

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