The invention relates to high-level production of pHBA in green plants
using a unique expression cassette. The latter comprises a chorismate
pyruvate lyase (CPL) coding sequence operably linked to a suitable
promoter capable of driving protein expression in higher plants.
Additionally, the CPL cassette comprises a sequence encoding a chloroplast
transit peptide, its natural cleavage site, and a small portion of the
transit peptide donor protein fused to the N-terminus of CPL. The
chloroplast targeting sequence targets the foreign protein to the
chloroplast compartment and aids in its uptake into the organelle. The
cleavage site is unique to the transit peptide, and cleavage of the
chimeric protein encoded by the cassette at this site releases a novel
polypeptide that has full enzyme activity, comprising the mature CPL
enzyme and a small portion of the transit peptide donor.