The subject invention pertains to methods and systems for testing a
substance for inflammatory or oxidant properties under acute inflammatory
conditions characterized by increased levels of redox-active metal ions.
The method includes the steps of applying an eccentric exercise stimulus
to a subject, thereby inducing a muscle injury; administering a substance
of interest to the subject; measuring one or more biological markers of
inflammation, oxidative stress, and muscle damage, or combinations
thereof, within the subject; and correlating the measured value of the
biological marker(s) with the inflammatory or oxidative properties of the
substance administered. The systems of the subject invention include means
for obtaining a biological sample from a subject, means for applying
eccentric exercise stimulus to the subject; means for measuring the amount
of the biological marker(s) within the biological sample; and means for
correlating the measured amounts of the biological marker(s) with the
inflammatory or oxidant properties of the substance administered.
