Method of determining substrate, and biosensor

   
   

A method of quantifying a substrate, by which the substrate contained in various samples can be conveniently and quickly quantified without resort to any troublesome pretreatments, and a biosensor. More particularly speaking, a method of quantifying a substrate in a sample by using an electrode system made of electrically conductive materials and a reaction reagent comprising at least a dehydrogenase, a coenzyme an electron mediator and a tetrazolium salt, which comprises performing an enzyme reaction and a redox reaction between the reaction reagent and the substrate in the sample, and detecting a formazan formed as the final reaction product by using the electrode system and a if biosensor with the use of the same are provided.

_ um método quantifying um carcaça, que carcaça contained vário amostra poss est conveniente e rápido quantified sem recurso algum incômodo pretreatments, e um biosensor. _ mais particular speaking, um método quantifying um carcaça um amostra using um elétrodo sistema faç elétrico condutor material e um reação reagent comprising menos um dehydrogenase, um coenzyme um elétron mediator e um tetrazolium sal, que compreend performing um enzyme reação e um redox reação entre reação reagent e carcaça amostra, e detecting um formazan d forma como final reação produto using elétrodo sistema e um se biosensor com us mesmo est forneç. _

 
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