The invention concerns a method for the determination of alkaline
phosphatase in a sample by optical measurement in which interference by
free haemoglobin or blood substitutes is eliminated by means of certain
wavelength combinations, a method for eliminating interference caused by
free haemoglobin or blood substitutes in a determination of alkaline
phosphatase and the use of certain wavelength combinations to eliminate
interference by free haemoglobin or blood substitutes.