A family of isolated and purified proteins and nucleic acids are disclosed.
Particularly, piwi family proteins and cDNAs encoding the same are
disclosed. Recombinant host cells, recombinant nucleic acids, recombinant
proteins and transgenic animals are also disclosed, along with methods of
producing each. Isolated and purified antibodies to piwi family homologs,
and methods of producing the same, are also disclosed. piwi family gene
products are characterized as having activity in the growth, proliferation
and self-renewing division of stem cells, and proliferation of primordial
germ cells. Thus, therapeutic, screening, culturing and transgenic methods
involving these activities are also disclosed.
Uma família de proteínas isoladas e purified e os ácidos nucleic são divulgados. Particularmente, as proteínas da família do piwi e DNAS que codificam o mesmo são divulgados. As pilhas de anfitrião recombinant, os ácidos nucleic recombinant, as proteínas recombinant e os animais transgenic são divulgados também, junto com métodos de produzir cada um. Os antibodies isolados e purified aos homologs da família do piwi, e os métodos de produzir o mesmo, são família também divulgada do piwi os produtos do gene que são caracterizados como tendo a atividade no crescimento, proliferation e divisão self-renewing de pilhas de haste, e proliferation de pilhas de germe primordial. Assim, therapeutic, selecionar, cultivar e métodos transgenic que envolvem estas atividades são divulgados também.
