Disclosed is a porous membrane built-in biosensor comprising (a) at least
one substrate; (b) an electrode layer patterned on the substrate,
consisting of an electrode system and a circuit connector; (c) an
insulator, formed on parts of the electrode layer, for electrically
separating the electrode system from a circuit connector; and (d) a porous
membrane via the insulator on the electrode system, wherein, when a whole
blood sample is introduced to the biosensor, the whole blood sample is
separated into its components during the chromatographic motion through
the porous membrane so that only blood plasma can be contacted with the
electrode system. The porous membrane built-in biosensor is provided with
a sample inlet, which allows samples to be introduced in a constant
quantity to the biosensors porous membranes without pretreatment.
