This invention provides methods, compositions and apparatus for mapping the
regulatory relationship among genes by massive parallel monitoring gene
expression. In some embodiments, mutations in the up-stream regulatory
genes are detected by monitoring the change in down-stream gene
expression. Similarly, the function of a specific mutation in a up-stream
gene is determined by monitoring the down-stream gene expression. In
addition, regulatory function of a target gene can be determined by
monitoring the expression of a large number of down-stream genes. The
invention also provides specific embodiments for detecting p53 functional
homozygous and heterozygous mutations and for determining the function of
p53 mutations.
