Purification of fibrinogen from milk by use of cation exchange chromatography

   
   

Fibrinogen is obtained from milk by contact with a cation exchange chromatography substrate under conditions where the fibrinogen binds to the substrate, followed by optional washing of the substrate. The bound fibrinogen is removed from the substrate by irrigation under increased ionic strength or increased pH conditions. The obtained fibrinogen may be naturally produced or transgenic.

Le fibrinogène est obtenu à partir du lait par le contact avec un substrat de chromatographie d'échange cationique dans des conditions où le fibrinogène lie au substrat, suivies du lavage facultatif du substrat. Le fibrinogène attaché est éliminé du substrat par irrigation dans la concentration ionique accrue ou les conditions accrues de pH. Le fibrinogène obtenu peut être naturellement produit ou transgénique.

 
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