The present invention provides compositions and methods for regulating
expression of heterologous nucleotide sequences in a plant. Compositions
include a novel nucleotide sequence for a vascular tissue-preferred
promoter for the gene encoding prunasin hydrolase and sequences isolated
therefrom. A method for expressing a heterologous nucleotide sequence in a
plant using the promoter sequences disclosed herein is provided. The
method comprises stably incorporating into the genome of a plant cell a
nucleotide sequence operably linked to the vascular tissue-preferred
promoter of the present invention and regenerating a stably transformed
plant that expresses the nucleotide sequence.