Novel methods for the synthesis of 8-nitroguanine are provided.
Compositions comprising 8-nitroguanine, made by the novel synthetic
methods are also provided herein. Methods of use of 8-nitroguanine, made
by the novel synthetic methods, as a standard for detection of
8-nitroguanine in samples are also encompassed within the scope of the
present invention. The present invention further concerns methods of
predicting organ transplant rejection and detecting exposure to
environmental stressors, such as ionizing radiation, toxic chemicals or
infectious agents, by detecting 8-nitroguanine in one or more samples from
a transplant recipient or an organism exposed to stress.
