Useful mutations of bacterial alkaline protease

   
   

The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.

A invenção atual relaciona-se aos mutations de um gene do subtilisin que resultam nas mudanças nas características químicas de enzymes do subtilisin. Os mutations em ácidos nucleic específicos do gene do subtilisin resultam em substituições do amino-ácido e conseqüentemente, função alterada do enzyme. Alguns destes enzymes do mutant exibem as propriedades físicas vantajosas às aplicações industriais, particularmente na indústria detergente, fornecendo o subtilisin que é mais estável à oxidação, possui uma atividade mais grande do protease, e exibe o washability melhorado.

 
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