An assay system using labeled probe molecules to identify and quantify
target molecules in a sample is disclosed. Where labeled probe molecules
are present on a substrate such as a microarray, the identification of
multiple different target molecules may be examined simultaneously.
Alternatively, a known number of like probe molecules may be present on a
substrate and a single target molecule may be quantified in a sample.
Preferred labeled probe molecules are comprised fluorescent single
stranded nucleotide analogs whose fluorescence is quenched by pairing with
a homologous nucleotide target sequence.
