In gene expression frequency analysis, a vector primer to which each cDNA
is ligated is formed by synthesizing the cDNA from each mRNA using by a
vector primer having a poly(T) sequence; each cDNA sequence is converted
to a tag on the vector primer; a concatemer is formed by ligating the
obtained tags via a sequence that enables recognition of ends of the tags;
and the nucleotide sequence of the concatemer is analyzed.
