Site-specific recombinase based methods for making a recombinant adenoviral
genome, as well as kits for practicing the same and the recombinant
adenovirus vectors produced thereby, are provided. In the subject methods,
the subject genomes are prepared from donor and acceptor vectors that each
include at least one site recombinase recognition site, where in certain
preferred embodiments, one of the donor and acceptor vectors includes a
single recombinase recognition site while the other includes two
recombinase recognition sites. The acceptor vector includes an adenoviral
genome having an E region deletion. The donor vector includes an insertion
nucleic acid. In the subject methods, the donor and acceptor vectors are
combined in the presence of a recombinase to produce an adenoviral genome
that includes the insertion nucleic acid. The subject adenoviral genomes
find use in a variety of applications, including as vectors for use in a
variety of applications.
