A method of treating CD33+ acute and chronic myeloid leukemia in animals
including humans, using fraction nos. 1 and 9 obtained from water:methanol
fraction by column chromatography, with ratio of water and methanol
ranging between 1:5 to 5:1, wherein said water:methanol fraction is
obtained from the polar extract of Piper betel by HPLC, with retention
time of 3.6 and 24.0 minutes respectively, with the fractions used both
individually, and in combination, and a composition including the fraction
nos. 1 and 9.