The present invention provides reconstituted and recombinant tissue
membranes and methods for pharmaceutical delivery of bioactive molecules.
In particular, reconstituted and recombinant amniotic membranes are
provided for sustained delivery of therapeutic molecules, proteins or
metabolites, to a site of a host in need thereof. The reconstituted and
recombinant amniotic membrane contains one or more recombinant expression
vectors that are exogenous to the membrane and capable of expressing
bioactive molecules. The reconstituted and recombinant tissue membranes
and methods can be used for in situ delivery of therapeutic proteins to a
host in the treatment of disorders such as chronic wounds and
dermatologic or ocular surface diseases.
