Peptide analogs the high molecular weight kininogen domain 3 are potent
inhibitors of angiogenesis. The peptides have the formula (a) X.sub.1
-Asn-Asn-Ala-Thr-Phe-Tyr-Phe-Lys-X.sub.2, (b) X.sub.3
-Cys-Val-Gly-Cys-X.sub.4, (c) X.sub.5 -Leu-Asp-X.sub.7
-Asn-Ala-Glu-Val-Tyr-X.sub.6, or (d)
Tyr-Phe-Ile-Asp-Phe-Val-Ala-Arg-Glu-Thr-Thr-X.sub.7 -Ser-Lys-Glu-Ser
wherein:
X.sub.1, X.sub.2, X.sub.3, X.sub.4, X.sub.5, and X.sub.6 are from zero to
twelve amino acids, independently the same or different, more preferably
from zero to six amino acids, and;
X.sub.7 is Ala or Cys.
The peptides may also comprise biologically active fragments of high
molecular weight kininogen domain 3. Methods of inhibiting endothelial
cell proliferation and angiogenesis are provided.
Os analogs do peptide o domínio elevado 3 do kininogen do peso molecular são inibidores potent do angiogenesis. Os peptides têm a fórmula (a) X.sub.1 - Asn-Asn-Ala-Thr-Phe-Tyr-Phe-Lys-X.sub.2, (b) X.sub.3 - Cys-Val-Gly-Cys-X.sub.4, (c) X.sub.5 - Leu-Asp-X.sub.7 - Asn-Ala-Glu-Val-Tyr-X.sub.6, ou (d) Tyr-Phe-Ile-Asp-Phe-Val-Ala-Arg-Glu-Thr-Thr-X.sub.7 - Ser-Lys-Glu-Ser wherein: X.sub.1, X.sub.2, X.sub.3, X.sub.4, X.sub.5, e X.sub.6 são zero a doze aminos-ácido, independentemente os mesmos ou diferente, mais preferivelmente zero a seis aminos-ácido, e; X.sub.7 é ala ou Cys. Os peptides podem também compreender fragmentos biològica ativos do domínio elevado 3 do kininogen do peso molecular. Os métodos de inibir o proliferation e o angiogenesis endothelial da pilha são fornecidos.
