This invention involves a process for selecting and producing eukaryotic alkaline
phosphatase in yeast. Yeast cells are subjected to multiple transformations using
a vector comprising a first resistance marker gene and the alkaline phosphatase
gene. Those strains that grow in media containing the first resistance marker are
further transformed using a vector comprising a second selection marker gene and
the alkaline phosphatase gene. Transformants that grow in media containing the
second selection marker are selected for expressing the eukaryotic alkaline phosphatase.
