An affinity matrix for use in affinity based molecular pull down and immunoprecipitation
procedures. The affinity matrix comprises a polymeric support, a dye attached to
a fraction of the polymeric support to enable optical detection of the polymeric
support, and an affinity ligand other than the dye attached to a fraction of the
polymeric support for the capture of a molecule.
Also provided is a method for the isolation of a biomolecule from an aqueous
solution. The method comprises combining the aqueous solution with an affinity
matrix comprising a polymeric support and separating the affinity matrix from the
aqueous solution. A dye is attached to a fraction of the polymeric support which
enables optical detection and monitoring of the affinity matrix and, accordingly,
reduces the likelihood of the loss of affinity matrix during the separation step.
In addition, an affinity ligand other than the dye is also attached to a fraction
of the polymeric support for the capture of the biomolecule.
