The invention relates to a process for genotyping any HCV isolate present in
a biological sample, previously identified as being HCV positive, and for classifying
said isolate according to the percentage of homology with other HCV isolates, comprising
the steps of:
- contacting said sample in which the ribonucleotides or deoxyribonucleotides
have been made accessible, if need be, under suitable denaturation, with at least
one probe from about 10 to about 40 nucleotides, with said probe being liable to
hybridize to a region being in the domain extending from nucleotide at position
-291 to nucleotide at position -66 of the 5 untranslated region of one
of the HCV isolates represented by their cDNA sequences, with said numbering of
position beginning with the first ATG codon of the open reading frame encoding
the HCV polyprotein, or with said probe being complementary to the above-defined probes,
- detecting the complexes possibly formed between said probe and the nucleotide
sequence of the HCV isolate to be identified.
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