The present invention provides a modified rapid expansion method (termed "low-PBMC-REM"
or "modified-REM"), for quickly generating large numbers of T lymphocytes, including
cytolytic and helper T lymphocytes, without using the large excesses of peripheral
blood mononuclear cells (PBMC) or EBV-transformed lymphoblastoid cells (LCL) characteristic
of high-PBMC-REM. Clonal expansions of greater than 500-fold can be achieved within
a single stimulation cycle of about 8-14 days.
