The invention provides a method for increasing production of a protein by cultured
mammalian cells comprising adding a DNA demethylating agent, for example a cytidine
analogue, to the mammalian cell culture. The cytidine analogue can be 5-aza-2-deoxycytidine
(also called decitabine) or 5-bromo-2-deoxycytidine-5-monophosphate,
among many other possible cytidine analogues.
