A method of treating particles to be used in immunoassays reduces interference
in particle agglutination assays. For particles having covalently bound antibodies
and residual NHS-ester or sNHS-ester groups on the surface, the reactive esters
are treated with an aqueous mixture containing an amine compound of formula (I):
The moiety X is NH2, OH, or CO2CH2CH3;
and R is an alkyl group or an alkyl ether group. When X is NH2
or CO2CH2CH3, R contains from 1 to 20
carbon atoms; and when X is OH, R contains from 4 to 20 carbon atoms.
