The present invention relates to the identification of novel serine
proteases in Gram-positive microorganisms. The present invention provides
the nuclei acid and amino acid sequences for the Bacillus subtilis serine
proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides
host cells having a mutation or deletion of part or all of the gene
encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides
host cells further comprising nucleic acid encoding desired heterologous
proteins such as enzymes. The present invention also provides a cleaning
composition comprising a serine protease of the present invention.
