Isolated recombinant polynucleotides comprising elements which promote encapsidation
into AAV particles, packaging cells comprising the recombinant polynucleotides,
and methods for their use are provided in the present invention. These isolated
recombinant polynucleotides comprise a non-AAV ITR encapsidation element (such
as the P1 sequence located within the AAV S1 integration site of human chromosome
19) operably linked to one or more heterologous genes to be encapsidated. The constructs
may be either integrated into a mammalian cell genome, maintained episomally, or
provided transiently.
