A device and process for the amplification of fluorescence emitted by an areal
sample. The device includes a support transmitting all or part of a fluorescence
signal and configured to support the areal sample and a thin layer interposed between
the support and the areal sample. The thin layer has a refractive index greater
than the refractive index of the support and than the refractive index of a medium
flooding the areal sample during a fluorescence measurement. The thickness of the
thin layer is chosen so that the thin layer transmits all or part of the fluorescence
signal which is measured after passing through the support.