The functional analysis of genes frequently requires the manipulation of large
genomic regions. A yeast-bacteria shuttle vector is described, that can be used
to clone large regions of DNA by homologous recombination. The important feature
of present invention is the presence of the a bacterial replication origin, which
allows large DNA insert capacity. The utility of this vector lies in its ability
to isolate, manipulate and maintain large fragments in bacteria and yeast, allowing
for mutagenesis by yeast genetics and simplified preparation of plasmid DNA in bacteria.
