Methods for improving binding of a proteinaceous substance to cell-wall material
of a Gram-positive bacterium are disclosed. The proteinaceous substance includes
an AcmA cell-wall binding domain, homolog or functional derivative thereof. The
method includes treating the cell-wall material with a solution capable of removing
a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from
the cell-wall material and contacting the proteinaceous substance with the cell-wall material.