A method for genetically modifying a target population of an organism,
comprising the steps of 1. providing a modified organism, wherein the
modified organism is capable of sexually reproducing with an organism of
the target population, and wherein a selected gene in the germline of the
modified organism is disrupted by having inserted into it a
sequence-specific nonMendelian selfish gene; 2. introducing the modified
organism into the target population. A method for genetically modifying a
target population of an organism, comprising the steps of 1. Introducing
a homing endonuclease gene (HEG) into the germline of an organism which
is capable of sexually reproducing with organisms of the target
population; 2. introducing the modified organism into the target
population. The HEG may encode a recombinant endonuclease, for example an
endonuclease with a zinc-finger DNA binding domain and a sequence
non-specific nuclease domain.
