With the object of providing a method for separating glycolipids
(particularly gangliosides) capable of processing a large number of
samples easily and at low cost, and capable of recovering multiple types
of glycolipids with high recovery, in the method for separating
glycolipids of the present invention (a) a sample solution obtained by
hydrolysis of an extract derived from a biological sample with a mixture
of a nonpolar solvent and a polar solvent is first brought into contact
via a semipermeable membrane with a solution having lower osmotic
pressure than the sample solution, and (b) the contact is continued until
the sample solution divides into two or three layers, and the middle
layer and/or bottom layer are/is separated.
