Ultrasound imaging of tissue in medical applications is carried out by
transmitting ultrasound energy into a target volume at least a first
fundamental frequency, receiving reflected and/or scattered ultrasound
energy from the target volume; and detecting components of the received
signal at multiple harmonics of the fundamental frequency or at one or
more of the third to fifth harmonics of the fundamental frequency. A
image of the target volume is generated using the detected harmonic
components of the received signal.