The invention relates to an optical system for determining the distribution,
environment, or activity of fluorescently labeled reporter molecules in cells for
the purpose of screening large numbers of compounds for specific biological activity.
The invention involves providing cells containing fluorescent reporter molecules
in an array of locations and scanning numerous cells in each location with a fluorescent
microscope, converting the optical information into digital data, and utilizing
the digital data to determine the distribution, environment or activity of the
fluorescently labeled reporter molecules in the cells. The array of locations may
be an industry standard 96 well or 384 well microtiter plate or a microplate which
is a microplate having a cells in a micropaterned array of locations. The invention
includes apparatus and computerized method for processing, displaying and storing
the data.
