A method of fabricating an elastomeric structure, comprising: forming a first
elastomeric
layer on top of a first micromachined mold, the first micromachined mold having
a first raised protrusion which forms a first recess extending along a bottom surface
of the first elastomeric layer; forming a second elastomeric layer on top of a
second micromachined mold, the second micromachined mold having a second raised
protrusion which forms a second recess extending along a bottom surface of the
second elastomeric layer; bonding the bottom surface of the second elastomeric
layer onto a top surface of the first elastomeric layer such that a control channel
forms in the second recess between the first and second elastomeric layers; and
positioning the first elastomeric layer on top of a planar substrate such that
a flow channel forms in the first recess between the first elastomeric layer and
the planar substrate.