The present invention relates to methods for producing a polypeptide, comprising:
(a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive
for the production of the polypeptide, wherein (i) the mutant cell comprises a
first nucleic acid sequence encoding the polypeptide and a second nucleic acid
sequence comprising a modification of at least one of the genes involved in the
production of a trichothecene and (ii) the mutant produces less trichothecene than
the parent filamentous fungal cell when cultured under the same conditions; and
(b) isolating the polypeptide from the cultivation medium. The present invention
also relates to mutants of filamentous fungal cells and methods for obtaining the
mutant cells, isolated trichodiene synthases and nucleic acid sequences encoding
the trichodiene synthases.
