The present invention provides a novel D-aminoacylase, as well as method for
producing a D-amino acid using the same. In order to achieve the above objective,
the present inventors have succeeded in purifying heat-stable D-aminoacylase from
microorganisms belonging to the genus Streptomyces by combining various
purification methods. Furthermore, the present inventors found that the purified
heat-stable D-aminoacylase is useful in industrial production of D-amino acids.
By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently
produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example,
N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine,
N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).
