The invention relates to an analytical chromatographic method which comprises
the steps of:
- a) providing a membrane type flow matrix attached to a liquid-impervious
backing, which flow matrix permits a capillary force assisted lateral fluid flow
therethrough, and at least a part of which flow matrix contains ion-exchange functions;
- b) treating the flow matrix to reduce or eliminate unspecific adsorption
properties of the flow matrix;
- c) applying to the flow matrix a sample containing at least two components;
- d) initiating a first lateral flow of aqueous fluid to transport the
sample through the flow matrix and separate said components therein;
- e) interrupting the lateral flow; and either
- f1) detecting at least one of the separated components on the flow matrix
in the position reached by the respective component when the flow was interrupted; or
- f2a) initiating a second flow of aqueous fluid to transport the components
in a direction substantially transverse to the direction of the first lateral flow;
- f2b) interrupting the second lateral flow; and
- f2c) detecting at least one of the separated components on the flow
matrix in the position reached by respective component when the flow was stopped.
The invention also relates to a chromatographic device comprising a membrane
type flow matrix attached to a liquid-impervious backing, which membrane permits
a capillary force assisted lateral fluid flow therethrough and at least a part
of which is modified to support ion-exchange functions.
