The present invention provides methods of producing a cloned non-human mammalian
nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal.
Embodiments of the methods include introducing donor genetic material into a metaphase
I oocyte; introducing donor genetic material into a non-enucleated oocyte; introducing
donor genetic material obtained from a donor cell that is at metaphase into an
oocyte; introducing donor genetic material into an oocyte, and naturally activating
the oocyte or the NT embryo; and introducing donor genetic material obtained from
a donor cell that is at late G1 phase into an oocyte.
