Provided are novel nucleic acid molecule encoding modified HAP1
DNA-binding domains. Also provided are novel reporter genes based on GFP
and extension. The materials provided by the invention may be used in a
variety of methods of activating genes having HAP1 upstream activation
sequences in plants, which methods can be used to co-ordinate or
investigate gene expression, optionally in conjunction with
GAL4-regulated expression, and also in novel "enhancer" traps.